Anti-Myelin Regulatory Factor (MRF)

Application

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Signaling

This Anti-Myelin Regulatory Factor (MRF) Antibody is validated for use in western blotting & IHC for the detection of Myelin Regulatory Factor (MRF).

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Myelin Regulatory Factor (MRF) in 10 µg of ARPE19 cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Myelin Regulatory Factor (MRF) in human cerebral cortex, human retina, and human cerebellum tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Myelin Regulatory Factor (MRF), also known as Myelin regulatory factor, MYRF, and encoded by the gene MYRF/C11orf9/KIAA0954/MRF, is an interesting protein that gets cleaved into two chains. The N terminal component specifically activates transcription of myelin gene in the CNS oligodendrocytes and pre-oligodendrocytes. The C-terminal portion, which is remains membrane bound, appears to play no role in gene induction but its complete role is still being discovered. The N-terminal portion of MRF activates MBP, MOG, MAG, and PLP among other genes, but it is not required for oligodendrocyte differentiation but is required for the maintenance of myelin in the adult. MRF whole factor is localized to the endoplasmic reticulum. The cleaved N terminal portion translocates to the nucleus and the C-terminal portion remains in the endoplasmic reticulum. Interestingly MRF can be found expressed outside of oligodendrocytes and oligo precursors in the brain including regions such as the lung, basal ganglion and liver.

Immunogen

Epitope: cytoplasmic domain, N-terminal chain

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human Myelin Regulatory Factor (MRF).

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Myelin Regulatory Factor (MRF) in 10 µg of mouse brain tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~160 kDa observed. The expected molecular weight is 125 kDa and ~160kDa when glycosylated. The protein was cleaved into a ~90 kDa N-terminal fragment from the full length of ~160 kDa